Case Studies
Novazone has proven that ozone application is successful in disinfecting, stopping or removing mold growth, reducing contamination
and decay, and improving shelf life.
Cold Storage Case-Lemons #1, Cold Storage Case-Lemons #2, Cold Storage Case-Table Grapes, Cold Storage Case-Kiwifruit, Disinfection Case-Apples, Disinfection Case-Canteloupes, Disinfection-Strawberries
COLD STORAGE CASE – LEMONS #1
A major citrus producer had excessive contamination in a cold room consisting of 175,000 cubic feet. Lemons contained over 3 million colony forming units of microorganisms per gram.
Ozone as a gas was applied for one night at specifications provided by Novazone.
Siliker Labs provided the independent testing. The results were widespread and substantial.
– Reduced contaminants by 2-3 log count
– Reduced ethylene-ripening losses
– Reduced mold spore-related losses
– Extended shelf life and improved product value
The table below depicts actual findings:
| |
Untreated |
Ozone Overnight |
Lemon: Aerobic Total Plate Count |
600 |
20 |
Yeast |
100 |
40 |
Mold |
3,000 |
70 |
Bin Dump Room: Aerobic Total Plate Count |
3,300,000 |
110,000 |
Yeast |
900,000 |
32,000 |
Mold |
400,000 |
70,000 |
| Source:Siliker Labs |
All units in:
Colony Forming Units per Gram |
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COLD STORAGE CASE – LEMONS #2
One of the largest packing associations in the world had significant losses from cross- contamination of mold spores.
Ozone was applied continuously in gaseous form over 100 days based on Novazone specifications within OSHA safety limitations. Novazone system monitored ozone levels.
Mold in the lemons treated with ozone was minimal, and was limited to the lemons that were infected prior to storage, as shown in the picture to the right.
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COLD STORAGE CASE – TABLE GRAPES
A premier grower of table grapes with multiple cold storage facilities in California’s San Joaquin Valley used sulfur dioxide in its cold storage rooms. The sulfur dioxide caused equipment corrosion in the grower's cold storage rooms, and still failed to prevent significant losses of its grapes to botrytis. The grower also planned to enter the organic market, where sulfur dioxide is prohibited by the USDA. Thus, they needed a safer and more effective cold storage treatment solution.
For conventional product, ozone was applied continuously in gaseous form within OSHA limits in conjunction with lower levels of SO2. For organic product, ozone alone was applied.
The results were widespread and substantial.
– Organic grapes thrived under ozone treatment (USDA Organic Rule in 2000)
– Reduced SO2 by two-thirds
– Reduced contaminants
– Reduced mold spore-related losses
– Extended shelf life and improved product value
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COLD STORAGE CASE – KIWIFRUIT
A multi-national packer with 60 years of operating history could no longer sustain 35 percent spoilage rates with kiwifruit. The targeted storage room in California contained 1,300 bins of kiwis.
Ozone was applied continuously in gaseous form based on Novazone specifications within OSHA worker safety limitations. Novazone system also monitored ozone levels.
The results were widespread and substantial.
– Spoilage reduced from 35% to less than 5%
– Reduced contaminants by 2-3 log count
– Reduced ethylene to non-detectable levels
– Reduced mold growth
– Extended shelf life
– Improved product quality
– Increased product value
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DISINFECTION CASE – APPLES
One of the largest caramel apple packaging facilities in the country is a Novazone customer. The apple treatment process used previously at this plant consisted of a recirculating flume of water that was chlorinated to control microorganisms. The flume had to be dumped daily, primarily because it accumulated high levels of organics and soil. Electronic Power Research Institute (EPRI) conducted a study for this company, in which ozone was applied in place of chlorine.
Apples were exposed to ozonated water for a short period of time.
The results were substantial and confirmed by EPRI.
– Water reusage increased from 1 day to 7 days
– Yeast and mold were reduced by 2-3 log count
– Shelf life was extended and average quality improved
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DISINFECTION CASE – CANTELOUPES
An international cantaloupe packer with a facility in Dominican Republic was confronted with disinfecting highly contaminated crops. Spoilage was significant due to the inability to effectively disinfect. Crops contained over 6 million colony forming units of microorganisms per gram
Cantaloupes were ozonated in two aqueous applications for a short period of time.
The results were substantial and confirmed by National Food Labs.
– Reduced contaminants by 3-4 log count
– Stopped mold growth
– Extended shelf life and improved average product quality
The table below depicts actual findings:
| |
Untreated |
Ozone Gas |
Aerobic Total Plate Count |
5,100,000 |
48,000 |
Coliforms |
11,000 |
50 |
E. coli |
<10 |
<10 |
Lactic Acid Bacteria |
5,100,000 |
45,000 |
Mold |
90,000 |
20 |
Yeast |
22,000 |
110 |
| Source:National Food Labs |
All units in:
Colony Forming Units per Gram |
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DISINFECTION – STRAWBERRIES
Novazone applied ozone gas for a short period of time to process harvested strawberries. Thereafter, both ozonated and treated strawberries were stored in separate but identical conditions.
The results after two weeks of storage were widespread and substantial.
– Reduced contaminants by 3-4 log count
– Untreated product was visually unacceptable
– Reduced mold and yeast growth to nominal levels
| |
Untreated |
Ozone Gas |
Aerobic Total Plate Count |
350,000 |
1,000 |
Coliforms |
160,000 |
90 |
E. coli |
<10 |
<10 |
Mold |
110,000 |
20 |
Yeast |
90,000 |
50 |
All units in:
Colony Forming Units per Gram
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